In vitro antibacterial effect of Argentine propolis extracts on Streptococcus mutans and Actinomyces viscosus

Aim: In the present study, antibacterial activity of Ethanol Extract Propolis (EEP), collected from various regions (Mendoza, Santiago del Estero, and Corrientes) in Argentina, against Streptococcus mutans ATCC® 35668™ Actinomyces viscosus 15987™ (MicroBioLogics Inc., USA) was investigated. Methods: Identification geographic botanical origin based on a reconnaissance survey. Phytochemical screening propolis carried out ethanolic extracts using standard methods to identify constituents (aluminum chloride colorimetric method, Folin-Ciocalteu thin layer chromatography). The agar diffusion method (discs wells) serial dilution (plates tubes) were used evaluate EEP. Results: EEP exerted degrees S. A. viscosus, depending area collection. showed that bioactive compounds correspond phenolic flavones. Tunuyán (Mendoza), where most abundant vegetation belongs Populus sp., highest content (220.92±2.01 mg/g) flavonoids (30.39±0.25 mg/g). This sample profound among tested. By agar-well we found high susceptibility with an inhibitory halo 11.25 ± 4.68 mm 10.90 4.21 respectively. It also presented low Minimum Inhibitory Concentration Bactericidal values (MIC 0.05 mg/mL - MBC 0.46 mg/mL) followed by 0.11 0.93 mg/mL). Conclusions: combined results all indicated is more susceptible effect than viscosus.Uniterms: Propolis. mutans. viscosus. Microbial sensitivity tests.